1. Purpose: Tricaine Methanesulfonate (MS-222) is a popular anesthetic agent used in aquatic species, and is intended for the temporary immobilization of fish, amphibians, and other aquatic, cold- blooded animals. 2.

All animals exposed to buffered MS222 displayed similar signs of distress as those of the animals exposed to unbuffered MS222. However, piping behavior, twitching, and erratic swimming were displayed by more animals than with the unbuffered solution, and none of the animals exposed to the buffered MS222 displayed rapid opercular movements. Prepare a stock buffered MS-222 solution as follows: a. weigh 0.08 g of MS-222 powder in a weighing “boat” b. weigh 0.16 g of sodium bicarbonate in a weighing “boat” c. add the weighed MS-222 and sodium bicarbonate to 1 litre of aged tap water, and mix thoroughly d. pour this buffered MS-222 solution into a labelled plastic bottle e. refrigerate this bottled stock MS-222 solution until needed (but within 1 month of preparation). The primary hazard of MS‐222 is that it is a respiratory irritant. Procedures Solid MS‐222 should be carefully weighed on the lab balance and dissolved in water/buffer at 10 to 1000 mg/liter (.001 to 0.1 percent) depending on species of fish. MS222 or TMS or Tricaine Methanesulfonate is used as an anesthetic and euthanasia agent in fish, amphibians and other cold-blooded animals. This document provides guidance regarding safety for personnel preparing MS222 solutions, the proper usage of the compound, dosages recommended for different applications and proper disposal. Apr 13, 2011 · Prolonged exposure (180 min) to a depressing dose of buffered MS-222 ® (25 mg/L) elevated cortisol more than an immobilizing dose (50 mg/L), while 100 mg/L was lethal within 30 min. Fish anesthetized (50 mg/L MS-222 ®) during a severe 30-min handling stress had significantly lower mortality than controls to a second handling stress applied Introduction: MS 222 is a common agent used for both fish and amphibious species (i.e. frogs) for temporary immobilization, anesthesia and euthanasia. Due to the acidic nature of MS 222, the solution must be prepared and buffered appropriately before use in any live, vertebrate aquatic species.

The hatching rate, mortality rate and malformation rate were manually counted every 24 hpf. After the larvae were anaesthetized with tris-buffered MS-222 (0.2 mg/ml, Sigma-Aldrich, Inc. St. Louis, Missouri, USA), the abnormal development of the embryo in different periods could be observed with stereoscopic microscopy.

• Topical buffered MS-222 • Blunt force trauma to the head • Benzocaine hydrochloride ¹Confirmation of death must include secondary method such as decapitation followed by pithing, double pithing, exsanguination, or chemical methods for destroying brain function. Immersion Anesthetics (Ex: Buffered MS-222) MS-222 is a basic compound and must be buffered using sodium bicarbonate. The resulting solution must be between pH 7 and 7.5.

Buffered Ms 222 Anesthetic, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more

• MS 222 - Buffered tricaine can be administered via water bath (amphibians), or injected directly into the lymph sacs (amphibians) or the coelomic cavity (amphibians and reptiles). - Dose for use in amphibians is 5 to 10 gm/L buffered with Na HCO3 to pH 7-7.5. Prolonged immersion for up to 1 hour may be necessary. Anesthetic overdose (MS‐222: Tricaine Methane Sulfonate) followed by pithing Animal is placed in a covered pan with 0.1% / 400‐500mg/L of a buffered MS‐222 solution until a level of surgical anesthesia is reached as verified by lack of response to a stimulus (toe pinch) prior to removal and Anesthetic overdose (MS‐222: Tricaine Methane  Sulfonate) and decapitation Fish are placed and left in a 250 ‐ 500mg/mL buffered MS‐222 solution for a minimum of 10 minutes after cessation of opercular movement (anesthesia to respiratory arrest) prior to removal and decapitation (indicate equipment used) to ensure euthanasia.